Target gene | Primer | Sequence 5’-3’ | Concentration (pmol) | PCR product size (bp) |
Shiga toxin 1 (stx1A) | LP30 | CAGTTAATGTCGTGGCGAAGG | 1.4 | 348 |
LP31 | CACCAGACAATGTAACCGCTG | |||
Shiga toxin 2 (stx2A) | LP43 | ATCCTATTCCCGGGAGTTTACG | 1 | 584 |
LP44 | GCGTCATCGTATACACAGGAGC | |||
Intimin (eaeA) | Int-Fc | CCGGAATTCGGGATCGATTACCGTCAT | 2 | 840 |
Int-Rc | CCCAAGCTTTTATTTATCAGCCTTAATCTC | |||
Bundle forming pilus (bfp) | Bfp-F | GGAAGTCAAATTCATGGGGGTAT | 1.5 | 254 |
Bfp-R | GGAATCAGACGCAGACTGGTAGT | |||
Flagelar antigen H7(fliCH7) | FLICH7-F | GCGCTGTCGAGTTCTATCGAGC | 2 | 625 |
FLICH7-R | CAACGGTGACTTTATCGCCATTCC | |||
Somátic antigen O157 (rfbO157) | PF8 | CGTGATGATGTTGAGTTG | 0.75 | 420 |
PR8 | AGATTGGTTGGCATTACTG | |||
Enterohemolysin (hlyA) | hlyA-F | GCATCATCAAGCGTACGTTCC | 4 | 534 |
hlyA-R | AATGAGCCAAGCTGGTTAAGCT |