| Target gene | Primer | Sequence 5’-3’ | Concentration (pmol) | PCR product size (bp) |
| Shiga toxin 1 (stx1A) | LP30 | CAGTTAATGTCGTGGCGAAGG | 1.4 | 348 |
| LP31 | CACCAGACAATGTAACCGCTG | |||
| Shiga toxin 2 (stx2A) | LP43 | ATCCTATTCCCGGGAGTTTACG | 1 | 584 |
| LP44 | GCGTCATCGTATACACAGGAGC | |||
| Intimin (eaeA) | Int-Fc | CCGGAATTCGGGATCGATTACCGTCAT | 2 | 840 |
| Int-Rc | CCCAAGCTTTTATTTATCAGCCTTAATCTC | |||
| Bundle forming pilus (bfp) | Bfp-F | GGAAGTCAAATTCATGGGGGTAT | 1.5 | 254 |
| Bfp-R | GGAATCAGACGCAGACTGGTAGT | |||
| Flagelar antigen H7(fliCH7) | FLICH7-F | GCGCTGTCGAGTTCTATCGAGC | 2 | 625 |
| FLICH7-R | CAACGGTGACTTTATCGCCATTCC | |||
| Somátic antigen O157 (rfbO157) | PF8 | CGTGATGATGTTGAGTTG | 0.75 | 420 |
| PR8 | AGATTGGTTGGCATTACTG | |||
| Enterohemolysin (hlyA) | hlyA-F | GCATCATCAAGCGTACGTTCC | 4 | 534 |
| hlyA-R | AATGAGCCAAGCTGGTTAAGCT |